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Table 1 The nanoparticle tracking analysis of EV-UC, EV-SEC, Protein-rich fraction, CM and CM-WO samples each derived from 3 different ASC donors

From: Adipose stromal cells bioproducts as cell-free therapies: manufacturing and therapeutic dose determine in vitro functionality

A. No particles/106 cells

ASC

EV-UC

EV-SEC

Protein rich fraction

CM

CM-WO

NanoSight

 Donor 1

6.38E + 06

5.79E + 08

1.06E + 08

1.57E + 07

1.06E + 08

 Donor 2

9.75E + 06

3.28E + 07

2.54E + 07

1.28E + 07

3.40E + 07

 Donor 3

4.67E + 06

2.35E + 08

1.65E + 08

1.81E + 07

2.88E + 07

ZetaView

 Donor 1

2.13E + 08

7.72E + 08

7.72E + 07

2.10E + 08

0

 Donor 2

7.08E + 08

7.83E + 08

2.17E + 07

3.00E + 08

0

 Donor 3

3.17E + 08

1.52E + 09

4.78E + 08

3.00E + 08

0

B. Particle diameter (nm)

ASC

EV-UC

EV-SEC

Protein rich fraction

CM

CM-WO

NanoSight

 Donor 1

123.6

–

135.9

98.0

165.5

 Donor 2

128.8

99.2

141.8

98.1

–

 Donor 3

113.7

179.6

124.8

160.3

122.9

ZetaView

 Donor 1

139.7

119.4

209.0

136.4

0

 Donor 2

190.2

308.5

273.2

118.8

0

 Donor 3

126.2

119.6

258.6

114.4

0

  1. The analysis was performed on samples derived from cell passage 4. A Number of particles derived from 106 ASC. B Mean particle diameter in nm. Data are shown as the mean of N = 3 ASC donors
  2. ASC - adipose stromal cells
  3. EV-UC - Extracellular vesicles isolated by ultracentrifugation
  4. EV-SEC - Extracellular vesicles isolated by size exclusion chromatography
  5. CM - Conditioned medium
  6. CM-WO - Conditioned medium wash-off
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Journal of Translational Medicine

ISSN: 1479-5876

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