Fig. 4

Enhancement of cell migration by ASC bioproducts. A Protein-Rich Fraction, CM and EV-UC preparations significantly enhanced endothelial cell migration with respect to the negative control (EndoGro-LS medium without FBS and VEGF, positive control with FBS and VEGF) in an in vitro wound healing model at 8 h after injury. CM and Protein-Rich Fraction displayed higher abilities to promote cell migration than any other treatment. B Migratory capacity of ASC 24 h after injury showed a tendency to increase after the addition of CM respect to the negative control (MEM-⍺ media without FBS) and positive control (MEM-⍺ media with FBS) (C) Increasing the ratio of EV:MSC leads to a significant increase in cell migratory capacity of EV-UC. Data are represented as the mean ± SD of N = 3 ASC donors and n = 6 technical replicates. Statistical analysis was performed using Two-Way ANOVA with Tukey’s multiple comparison test; * = p < 0.05, ** = p < 0.001, *** = p < 0.0001, **** = p < 0.00001. # Significance versus the negative control