Fig. 1

Scheme of isolation of ASC bioproducts. The ASC were cultured in traditional 2D culture flasks in serum-free medium for the production of EV-UC (A). The supernatant was collected and then ultracentrifuged for 2 h at 100,000 × g, the EV-UC were collected, cryopreserved and shared between the centers. For the production of EV-SEC (B), the ASC were cultured in a 3D hollow fiber bioreactor (HFBR), from which the supernatant was collected and concentrated with 100 kDa filter. The samples were then processed with size exclusion chromatography and the EVs and protein fraction were concentrated with a 100 kDa filter and collected. The EV-SEC and the protein-rich fraction were shared between centers. Finally, the ASC were cultured in traditional 2D culture flasks, the supernatant was collected and concentrated with 3 kDa and the conditioned medium and wash-off were collected during the process (C), cryopreserved and shared between centers