Fig. 1
From: Ligand-based adoptive T cell targeting CA125 in ovarian cancer
Construction and detection of CR and CAR expressed T cells. A The original structure of chimeric antigen receptor (CAR) and chimeric receptor (CR) targeting CA125; B the structure of CR and CAR-expressing plasmids; C flow cytometry detection of the CA125 binding activity of T cells transduced with CR, CAR, and CR + CAR; D the Kd value of CR, CAR and CR + CAR comparation targeting CA125 protein. E qPCR results of mRNA expression levels of electro-transduced CR, CAR, and CR + CAR expressed T cells group after 24 h. All data are presented as the mean ± SEM of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, P > 0.05 labeled as N.S was considered no significance