Fig. 6

Cortical ATF4 and eukaryotic translation initiation factor 2 Alpha (eIF2α) at the mRNA level and phosphorylated (eIF2α ~ P) at the protein level. A Brain cortical ATF4-mRNA expression (N = 6–7 per group). B Brain cortical eIF2α-mRNA expression (N = 5–6 per group). C Representative merged images of DAPI—DNA blue staining of cells' nuclei and eIF2α ~ P protein-red staining in SBN/y and SBH/y groups at three-time points: Baseline, 6 months, and 9 months after RD or HSD. Scale bar: 50 µm. D IF analysis of the cortical eIF2α ~ P protein expression (N = 6–7 per group). Mean ± SEM. Three-way ANOVA was followed by Tukey’s multiple comparison test. ^**P < 0.005; ^***P < 0.001