Fig. 5
From: Triple blockade of Ido-1, PD-L1 and MEK as a potential therapeutic strategy in NSCLC
A Localization by immunofluorescence of Ido-1 in patient-derived tumour spheroids. Images represent different tumour spheroids deriving from the same patient sample: 4′,6-diamidino-2-phenylindolefluorescent nuclear staining (blue), Ido-1 staining (green). Scale bar: 10 µm. B MTS cell proliferation assay in human spheroids (n = 5) after 6 days of treatment with MEK-inhibitor, selumetinib, anti-PD-L1 drug, atezolizumab, and Ido-1 inhibitor as single agents and in combination. One-way ANOVA test followed by Tukey’s test were used for statistical analysis **p < 0.01. C Representative images of one sample. D Effect of drug treatment on induction of apoptosis in three dimensional spheroid cultures. Representative flow cytometric analysis of apoptosis. One representative experiment is shown. E Histogram of data expressed as median percentage of apoptotic cells in 5 samples