Fig. 4
From: Triple blockade of Ido-1, PD-L1 and MEK as a potential therapeutic strategy in NSCLC
A Real time qPCR analysis of IFNγ, TNFα, IL-6 and IL-12 expressed by mRNA from PBMC before (Control) and after treatment with selumetinib (Mek-I), Atezolizumab (ant-PD-L1) alone and in combination. Results were normalized to 18S mRNA and analyzed by ΔCt method. One-way ANOVA test followed by Tukey’s test were used for statistical analysis (** p < 0.01). B Flow cytometry analysis of immune checkpoint molecules as PD-1/PD-L1, Ido-1, CTLA-4, TIM3 and LAG3 in PBMCs surface before (Control) and after treatment with selumetinib plus atezolizumab, Ido-1 inhibitor (anti-Ido-1) alone and in triple combination