Figure 3
![Figure 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1479-5876-10-21/MediaObjects/12967_2011_Article_994_Fig3_HTML.jpg)
Increasing DDP induced apoptosis after ATP7A's silence for 80 h by siRNA in A549/DDP cells. Apoptotic or necrotic cell death was tested by flow cytometric analysis. Control siRNA, added 100 nM nonsilencing siRNA sequences for 8 h and then added DDP for 72 h; ATP7A siRNA, added 100 nM SiRNA targeting ATP7A for 8 h and then added DDP for 72 h. Columns, mean of three independent experiments; bars, SD.